solved. Question
Answered step-by-step
Asked by josephinejose
Nursing 2007; Answer if sure
1.   Upon keen analysis of the results in this article do you think saturation of research was arrived upon? https://www.ncbi.nlm.nih.gov/books/NBK148970/
2.   Discuss keenly whether reflexivity was achieved?
3.   What was the researcher’s level of significance level?
4.   Choose the correct statement for the lambda ZAP vector.
a) It is not based on bacteriophage lambda
b) It contains a region that can be excised in vivo
c) The excision leads to the formation of bluescript plasmids and it contains an initiator region only
d) The multiple cloning site is not flanked by the initiator and the terminator region
5.   The initiator is recognized by which gene?
6.   Choose the incorrect statement for the replication process.
a) The initiator site is nicked and replication of one strand is started
b) Replication takes place in both the directions
c) Replication continues through the bluescript
d) It is stopped at the terminator and then again a nick is made
7.   Which of the following doesn’t takes place after replication?
a) The single stranded sequence is generated
b) It is circularized to form closed single stranded molecule
c) It may circularize or remain linear
d) The double stranded molecule can be synthesized by cellular DNA synthesis
8.   Choose the correct statement for cosmids.
a) It can be regarded as lambda substitution vector
b) Less amount of phage DNA is deleted
c) Only cos packaging sites are left
d) It doesn’t contains a origin of replication
9.   Once cosmids are inside the E.coli cells, they don’t generate phage but are propagated as plasmids.
a) True
b) False
10. Which of the following is the correct method to check whether the DNA has entered into the cell or not in the case of cosmid?
a) If a transformation has taken place turbid plaques are formed
b) If a transformation has taken place clear plaques are formed
c) If a transformation has taken place, it can be confirmed via ampicillin resistance
d) If a transformation has taken place, it can be confirmed if forms plaques and is ampicillin resistant also
11. Which size of the insert is accepted by the cosmids?
a) 10-20 kbp
b) 35-45 kbp
c) 50-60 kbp
d) 100-120 kbp
12.  What happens once the cosmid enters the E. coli cells?
a) There is strict size selection inside E. coli cells
b) Partial deletion may take place
c) The tendency of deletion may is increased by using low copy number
d) The tendency of deletion can’t be altered
SCIENCE
HEALTH SCIENCE
NURSING
